EFFECT OF ASPIRIN ON OXIDATIVE STRESS STATUS OF LIVER AND KIDNEY TISSUES OF WISTAR RATS

Abstract

Aspirin has been linked to inflammatory changes in liver and kidney, but it is not clear whether this effect is
associated with Aspirin-induced oxidative stress in these organs. The present investigation was therefore aimed at
determining the effect of Aspirin on oxidative stress markers in the serum, liver and kidney tissues of Wistar rats.
Acute Aspirin had no effect in serum Malondialdehyde (MDA) level, Superoxide Dismutase (SOD) activity and
Glutathione reductase (GSHRd) activity; however serum Catalase (CAT) activity and serum reduced glutathione
(GSH) level were significantly (p<0.05) decreased. Sub-acute Aspirin significantly (p<0.05) decreased serum MDA
level and CAT activity but had no effect in SOD activity, GSH level and GSHRd activity. In the liver tissues, acute
Aspirin significantly (p<0.05) decreased MDA level, GSHRd activity and SOD activity but had no effect in GSH
level and CAT activity. Meanwhile sub-acute Aspirin in the liver significantly (p<0.05) decreased MDA level, CAT
activity and GSH level but had no effect in SOD activity and GSHRd activity. In the kidney tissues, acute Aspirin
had no effect in MDA level; CAT activity, SOD activity and GSH level but GSHRd activity was significantly
(p<0.05) increased. However, sub-acute Aspirin significantly (p<0.05) increased the kidney’s MDA level, CAT
activity and SOD activity while GSH level and GSHRd activity were significantly (p<0.05) decreased. Acute
administration of Vitamins C and E prior to Aspirin as well as vitamins alone significantly (p<0.05) decreased MDA
level in all the tissues while CAT activity, GSH level and GSHRd activity were significantly (p<0.05) increased in
the liver and kidney tissues. Sub-acute administration of vitamins prior to Aspirin significantly (p<0.05) increased
MDA level in serum and liver tissues unlike in the kidney tissues, while CAT and SOD activities were not affected
in all the tissues. In conclusion Aspirin prevent oxidative tissue damage in the serum and liver by decreases in MDA
levels and modulation of CAT and SOD activities, but in the kidney Aspirin caused oxidative damage by increased
MDA level, CAT and SOD activities.