DNA BARCODING AND HPLC–DAD–ESI-MS/MS CHARACTERIZATION OF COMPOUNDS FROM COMMIPHORA AFRICANA (A. RICH.) ENGL. (BURSERACEAE)

Authors

  • A. Z. Abubakar Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria
  • A. Adamu Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria
  • U. F. Shehu Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria
  • S. Shehu Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria
  • A. Abubakar Department of Pharmacology and Therapeutics, Ahmadu Bello University, Zaria
  • G. Ibrahim Department of Pharmacognosy and Drug Development, Ahmadu Bello University, Zaria

Keywords:

DNA barcoding, Commiphora africana, BLAST, rbcl.

Abstract

DNA barcoding is a novel method of species identification based on nucleotide diversity of conserved sequences. It
provides authentic identification of plants on a molecular basis. Commiphora africana is a famous medicinal plant
used traditionally in the treatment of various ailments such as inflammation, stomach disorders, diabetes, malaria,
tumors, skin infections and as antidotes for venomous bites and stings in Nigeria and other parts of Africa. The
present study aims to develop DNA barcodes and chemical profile for C. africana. The total genomic DNA was
extracted from the young leaves of C. africana, the isolated total genomic DNA was PCR amplified using the rbcl
and matk markers and the PCR products were resolved on agarose gel electrophoresis. The amplified band was
sequenced and analyzed using Basic Local Alignment Search Tool (BLAST). The LC/MS was carried out on the C.
africana stem bark ethanol extract through HPLC–DAD–ESI-MS/MS experiments. The primer (rbcl) gave good
amplification and produced a barcode region with 550 bp in this plant species while the matk marker could not
produce a visible band. About 10 chemical compounds were tentatively identified from the stem bark of C. africana.
The results showed that the use of these conserved DNA sequences as barcodes and chemical profiles would be an
accurate way for species identification and discrimination.

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Published

2023-10-31

Issue

Vol. 22 No. 2 (2023)

Section

Articles