CYTOTOXIC ACTIVITY OF ISOLATES FROM TETRAPLEURA TETRAPTERA TAUBERT (MIMOSACEAE) POD AGAINST BREAST CANCER CELLS AND LEISHMANIA MAJOR PROMASTIGOTES
Keywords:
Breast cancer, Cytotoxic, Leishmanicidal, Tetrapleura tetraptera,Abstract
The cytotoxic effects of Tetrapleura tetraptera pod in vitro and in vivo has been reported. This work examined
the cytotoxic effect of its compounds obtained through Vacuum Liquid chromatography, Column
chromatography and High-Pressure Liquid chromatography. Compounds TT2 and TRA obtained were tested on
AU565 and MDA-MB231 breast cancer cell lines respectively, at 50 μM, and analysed with 3-(4, 5-
dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay. Antileishmanial activity of compound
TRA on Leishmania major promastigotes was also examined. TT2 and TRA gave +14.36 and +19 % inhibitions
on AU565 and MDA-MB231 breast cancer cells respectively. Significant activity was observed on L. major
with compound TRA, having an IC50 value of 35.04 ± 0.17 μM. On the basis of the mass spectrometric (MS)
data and comparison of 1H and 13C-NMR data, the compounds TT2 and TRA were identified as 22-
hydroxyisohopane and 3-O-[β-D-glucopyranosyl-2'-acetamido-2'- deoxy]-oleanolic acid (aridanin) respectively.
These results show the potential of this plant for usage against these diseases.