ROLE OF VIRGIN COCONUT OIL ON MALE REPRODUCTIVE FUNCTIONS IN RATS ADMINISTERED L-NAME

Abstract

Nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) has been reported to diminish sperm
functions, reduce fertility, and affect male sexual function. Both nitric oxide (NO) and essential oils are known to
stimulate sperm motility. We investigated the role of virgin coconut oil (VCO) on sperm functions and biochemical
parameters in rats administered L-NAME. Twenty-four male rats weighing 90–100 g were divided into four groups
of 6 rats each. Group I – control, Group II – L-NAME (50 mg/kg b.w), Group III – L-NAME + 10% VCO enriched
diet, and Group IV – 10% VCO enriched diet only. Rats were fed for 3 weeks, and the caudal epididymis was used
for the assessment of sperm parameters. Blood samples were withdrawn to assay biochemical parameters. Sperm
count and motility were significantly reduced in rats administered L-NAME and L-NAME + VCO. VCO
supplementation with L-NAME increased testosterone and nitric oxide levels that were decreased by L-NAME (p<
0.05). The cardiac risk ratio was increased in L-NAME-administered rats. Malondialdehyde level was increased in
the L-NAME group while the antioxidant enzymes reduced glutathione, superoxide dismutase and catalase activities
were reduced. However, VCO supplementation reduced the effect of L-NAME on malondialdehyde and increased the
antioxidant enzymes. The weights of the animals remained the same even though there was an increase in food
consumption during the first week of supplementation in the VCO-supplemented rats. VCO attenuated the adverse
effects of L-NAME on lipid profile, nitric oxide, antioxidant status, and testosterone in male rats.