ISOLATION AND CHARACTERIZATION OF TRITERPENOID AND TWO PHYTOSTEROLS FROM THE ROOT OF FICUS ITEOPHYLLA LINN (MORACEAE)

Abstract

This study reports the isolation and characterization of some bioactive compounds from the root of Ficus iteophylla.
Powdered root of the plant was extracted with hexane and was subjected to chromatographic separation over a silica
gel G (50–200) and preparative thin layer chromatography. Three compounds were isolated and coded as AMX, AM
and F4. In 1H-NMR of AMX, H-3 proton appeared as a doublet of doublets at δH 3.20 ppm, two broad singlets at
δH4.57 ppm and 4.69 ppm, seven methyl singles at δH 0.78 ppm, 0.85 ppm, 0.95 ppm, 0.98 ppm, 1.01 ppm, 1.21 ppm,
1.69 ppm and 13C-NMR revealed 30 signals typical for lupane skeleton. The AM 1H-NMR showed H-3 proton as
multiplets at δH 3.50 ppm, H-6 as singlet at δH 5.36 ppm, six methyl protons appeared as two three protons singlets at
δH 1.01 ppm and 1.25 ppm, one three protons broad singlet at δH 1.59 ppm, two doublets for three protons at δH 0.69
ppm and 0.92 ppm, one doublet of triplets for three protons at δH 0.83 ppm and 13C-NMR revealed the signals for all
the twenty-nine carbon atoms. The 1H-NMR of F4 revealed six methyl protons as two methyl singlets at δH 0.70 ppm
and 1.08 ppm, two methyl doublets at δH 0.85 ppm and 1.02 ppm, two triplets at δH 1.16 ppm and 0.80 ppm, the H-3
proton appeared as multiplets at δH 3.52 ppm, three olefinic protons at δH 5.35 ppm (d), 5.02 ppm (dd), 5.15 ppm (dd)
and the 13C-NMR revealed twenty nine carbon signals. From physical, chemical and spectral characterization of AMX,
AM and F4 were concluded as Lup-20(29)-ene-3-ol, Stigmat-5-ene-3-ol and Stigmat-5, 22-diene-3-ol respectively.