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Journal Of Tropical Biosciences

Year: 2016|   Volume No: 11|   ISSN: 0795-3089|   Page No: 135


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Title:  EVALUATION OF THE ANTIOXIDANT ACTIVITY OF ETHYL ACETATE FRACTION OF NIGELLA SATIVA (L.) SEED EXTRACT ON GASTRIC MUCOSAL INTEGRITY IN RATS

IBRAHIM S.M.A1,MABROUKS, M.A1., MUSA, K.Y2. MOHAMMED1, A., ALHASSAN, A.W1, ISA, A.I1 AND HEBA, D.3

1DEPARTMENT OF HUMAN PHYSIOLOGY, FACULTY OF MEDICINE, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA.
2DEPARTMENT OF PHARMACOGNOCY AND DRUG DEVELOPMENT, FACULTY OF PHARMACEUTICAL SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA
3ANIMAL SCIENCE UNIT, ELKASR EL-AIN, CAIRO UNIVERSITY, CAIRO EGYPT

ABSTRACT

Nigella sativa L. is one of the most extensively studied plants both phytochemically and pharmacologically. It is an annual flowering plant native to Southwest Asia and indigenous to the Mediterranean region, Middle-East, but now widely found in India. In the UnaniTibb system of medicine, N. sativa is regarded as a valuable remedy for a number of diseases including gastric diseases. The present study was carried out to investigate the gastroprotective and antioxidant effect of Ethyl Acetate (ETAC) fraction of Nigella sativa L. (Family: Ranunculaceae) seed extract on indomethacin-induced gastric ulcer model in adult male albino wistar rats. Phytochemical screening of the fraction (Trease and Evans, 2002) revealed significant and variations in the presence of flavonoids, alkaloids, tannins, saponins, steroids/triterpenes, glycosides/glucosinolates and free anthraquinones in the fraction, while acute toxicity study (Lorke’s Method,1983) revealed a lethal dose (LD50) above 5000 mg/kg. Animals were divided into 9 groups (n=5). Indomethacin 20mg/kg was used as the necrotizing agent, while cimetidine at a dose of 100mg/kg was used as standard drug and normal saline 1ml/kg as negative control. The extract was administered subcutaneously thirty minutes prior to indomethacin (20 mg/kg) at doses of 50, 100 and 200 mg/kg. The various parameters studied were malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activities. Pretreatment with the fractions at doses of 50 and 100 and 200mg/kg led to a significant increase in CAT and SOD activities, while MDA concentration significantly (p<0.05) decreased compared to the control group. This inhibition of lipid peroxidation and the generation of MDA and related substances from lipids that react with thiobarbituric acid were found to be inhibited by this extract. Thus, it appears that the anti-oxidant property of this extract could counteract oxidative damage caused by NSAIDs. In conclusion, the gastroprotective properties of this fraction evaluated may be attributed to its reducing effect on oxidative damage and neutrophil infiltration in tissues due to the presence of phytochemicals like flavonoids, alkaloids and tannins amongst others present in the seed extracts with various antioxidant and other biological activities

SUMMARY:

These studies suggest that the ETAC fraction of Nigella sativa protects against free radicals and possess appreciable free radical scavenging property by promoting oxidant/antioxidant
balance, hence it might play a role in combating gastric ailments like peptic ulcers

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