Journal Of Tropical Biosciences
Volume No: 11|
Page No: 118
| FullText PDF
Title: IN VITRO AND IN VIVO ANTITRYPANOSOMAL ACTIVITY OF GANODERMA LUCIDUM AND LENZITES BETULINA
1*G.A. JEROME, 1I.S. NDAMS, S.A. LUKA, 3J.A. ABEDO AND 2A.I. OKAFOR
1DEPARTMENT OF BIOLOGICAL SCIENCES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
2DEPARTMENT OF BIOCHEMISTRY, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
The preliminary in vitro test using hexane, ethyl acetate, methanol and aqueous extract of the mushrooms showed that the aqueous extract were the most potent followed by methanol, ethyl acetate and hexane respectively. The in vitro activity of the most potent aqueous extract of the mushrooms and (DiminalR) revealed that doses of 0.4g/mL, 0.2g/mL and 0.1g/mL had effects against the parasites. The LD50 of the extract showed that the mushrooms were not toxic. Healthy mice infected with 0.02mL of the inoculums, showed noticeable increase in parasitemia by day 3 post infections, the parasites were seen in the blood films and gradually increased till the 7th day post infection before the death of the animals on the 8th day post infection. There were no significant differences between the mice treated orally, suppressive and prophylactic in both mushrooms. Only the Diminal treated extract completely cleared the parasite on the 4th day post infection, Parasitaemia level increased with decrease in body weight and decrease in PCV in the extract treated controls, the positive and solvent treated control mice all survived with gradual in increase in PCV. While in the negative control group Parasitaemia level greatly increased.
Aqueous extract of the mushrooms displayed little or no activity against the parasite in the mice treated orally, prophylactic and suppressive. The trypanosomes in the blood of
the treated mice increased until the 6th and 7th day post infection and massive death was
recorded on the 7thday. Parasitaemia level increased with decrease in body weight and
decrease in PCV in the extract treated controls. But the positive controls mice all survived with gradual decrease in PCV on the 2nd day post treatment and increase in PCV in Solvent treated group. Parasites were not detected in their bloodstream by microscopic examination. The level of parasitaemia in the negative control group (infected not treated) greatly increased. They also showed a decrease in body weight and PCV reading and all of the mice died by day 7 post infections.