Journal Of Tropical Biosciences
Volume No: 11|
Page No: 99
| FullText PDF
Title: EFFECTS OF PAPAIN ENZYME TREATMENT ON THE TENDERNESS OF BEEF FROM THE CHUCK CUT OF SPENT COW
E. Z. JIYA; O. B. OCHEME; G. MUSA AND O. J. ALABI
DEPARTMENT OF ANIMAL PRODUCTION, FEDERAL UNIVERSITY OF TECHNOLOGY MINNA
An experiment was conducted to investigate the effects of papain enzyme concentration and cooking time on beef tenderness from the chuck meat of spent cow. The papain enzyme was obtained from precipitate that settled out from a two-carbon organic co-solvent system which comprised 77% equal volume of ethanol and acetone and 23% pawpaw fruit liquid extract (crude protein solution). Uniformed-sized of beef from the chuck portion of spent cow was marinated with different concentrations of papain extract (0 ml, 10 ml, 15 ml), aged in the freezer and cooked at 75oC for 30 and 60 minutes in a water bath. Data was collected on physico-chemical properties of the meat as well as the sensory evaluation. Cooking time and levels of inclusion of papain enzyme significantly (P<0.05) affected the meat quality characteristics measured. There were interactions between cooking time and levels of enzyme inclusion in the results of water holding capacity and pH. The amino acid content, mineral and the sensory evaluation of the chuck meat were all significantly (P<0.05) affected by the cooking time, levels of inclusion as well as interactions between cooking time and enzyme inclusion levels. The result showed that the papain extract derived from the two-carbon organic co-solvent extraction of pawpaw fruit could be used as an effective meat tenderizer within a short cooking time. It was concluded that papain enzyme treated beef from the chuck of spent cow can be cooked for 30 minutes with up to 15mls level of inclusion for better meat tenderization.
The result of this study showed that cooking time with papain enzyme inclusion levels tenderized meat without affecting other quality parameters adversely. Thus papain enzyme treated beef from the chuck of spent cow can be cooked for 30 minutes with up to 15mls level of inclusion for better meat tenderization.