ANTIBIOTIC RESISTANT PROFILE OF COAGULASE POSITIVE STAPHYLOCOCCUS SPECIES AMONG CLINICAL ISOLATES IN ZARIA METROPOLIS, KADUNA

Abstract

Coagulase positive Staphylococci (CoPS) are usually pathogenic causing diseases ranging from mild to severe.
This study was to determine the occurrence and antimicrobial resistant profile of coagulase positive
Staphylococci (CoPS) from different clinical source. A total of 150 presumptive coagulase positive
staphylococcal isolates from all specimens, submitted to the laboratory microbiology unit of the hospitals were
collected, and screened using Microbiology standards. Disc agar diffusion method was used for eleven (11)
antibiotics susceptibility test while vancomycin screening agar and Broth Micro dilution test was used for
vancomycin susceptibility evaluation. Biofilm assessment was performed using congo red agar (CRA) and
Microtitre plate assay method. Percentage Occurrence of coagulase positive reveals S aureus (21%), S
intermedius (15%), S pseudintermedius (7%), S hyicus (27%), S schleiferi (4%), S lutrae (14%). Antibiotic
susceptibility testing revealed resistance pattern ranging from 18 to 78%. With Non MDR 14(19%), MDR
44(60%), XDR 13 (18%) and PDR 2(18%). Phenotypic evaluations showed 75% strains to be MRCoPS, 37% as
VRCoPS, and 22% as both MRCoPS and VRCoPS. Biofilm strains revealed 66% and 75% of isolates using
CRA and Microtitre plate assay respectively. The emergence of MDR has resulted to ineffective therapy. An
urgent therapeutic strategy is needed in our clinical settings to stem the tide of difficult to treat infections.