HEPATO-CARDIO PROTECTIVE POTENTIAL OF ALPHA LIPOIC ACID IN 5- FLUOROURACIL-INDUCED TOXICITY OF WISTAR RATS

Abstract

The aim of this study was to assess the protective potential of Alpha Lipoic Acid in 5-Fluorouracil induced
hepato-cardio toxicity in Wistar rats. Wistar rats were randomly divided into six different groups (n=6 each).
The first group received normal saline (1ml/kg) for four days. The second group received a daily dose of
(1ml/kg) normal saline and 5-fluorouracil (50 mg/kg), orally for four days. The third group received Silymarin
100 mg/kg orally for four days. The fourth group received a daily dose of alpha lipoic acid (ALA), 100 mg/kg
orally, and the fifth group received a daily dose of ALA (200 mg/kg, orally) for four days. The Silymarin and
alpha lipoic acid treatments were given an hour before 5-fluorouracil administration. The sixth group received
5-fluorouracil (50 mg/kg, orally) for four days and 400mg/kg alpha lipoic acid for 14 days. All the experimental
animals were sacrificed at the end of the experimental procedures. Single intraperitoneal injection of 50 mgkg-1
of 5-FU was associated with significant (p˂0.05) increases in the malondialdehyde (MDA), lactose
dehydrogenase (LDH) and creatinine phosphokinase (CPK) levels in the toxicity model control when
compared with untreated control rats. However, a single intraperitoneal injection of 50 mg/kg 5-FU was
associated with significant (p˂0.05) decrease in the Glutathione (GSH), Superoxide Dismutase (SOD), Catalase
(CAT), Glutathione Peroxidase (GPx), levels in the toxicity model control when compared with untreated
control rats. Pretreatment and post treatment with ALA attenuated these changes.